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Fast immunoassay methods and methods
Rapid immunoassays take advantage of the fact that antibodies are unable to bind to the hydrophobic surface of an unwetted Immobilon®-P membrane, but can effectively recognize and bind to proteins that have been immobilized on the membrane. These assays are compatible with both chromogenic and chemiluminescent detection systems, making them versatile for a range of applications.
Tip: If you're looking to quickly detect high-abundance proteins, a rapid immunoassay is an excellent choice. However, for greater sensitivity and lower detection limits, standard immunoassays are typically more suitable.
One of the key benefits of using a rapid immunoassay is that it eliminates the need for blocking steps, which saves time and reduces the risk of errors or inconsistencies (Mansfield, 1994). Additionally, because excess antibody doesn't bind to the dried membrane, fewer washes are required, further streamlining the process. As a result, the entire procedure can be completed in under two hours, compared to over four hours for traditional methods.
Important: Make sure the blot is completely dry before beginning the rapid immunoassay. Any residual moisture may interfere with the binding efficiency and lead to unreliable results.
By simplifying the workflow without compromising accuracy, rapid immunoassays are ideal for researchers who need fast, reliable data without sacrificing quality. Whether you're running a quick check or optimizing your protocol, this method offers a practical alternative to conventional techniques.