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Method principle The soil is heated together with concentrated sulfuric acid and a reducing catalyst to convert organic nitrogen into ammonia and combine with sulfuric acid to form ammonium sulfate; inorganic ammonium nitrogen is converted into ammonium sulfate; a very small amount of nitrate nitrogen escapes during heating Loss; Organic matter is oxidized to CO2. After the sample is digested, it is distilled again with concentrated alkali to convert ammonium sulfate into ammonia to escape and be absorbed by boric acid, and finally titrated with standard acid. The main reaction can be expressed by the following equation:
NH2? CH2CO? NH-CH2COOH + H2SO4 = 2NH2-CH2COOH + SO2 + [O]
NH2-CH2COOH + 3H2SO4 = NH3 + 2CO2 ↑ + 3SO2 ↑ + 4H2O
2NH2-CH2COOH + 2K2Cr2O7 + 9H2SO4 = (NH4) 2SO4 + 2K2SO4 + 2Cr2 (SO4) 3 + 4CO2 ↑ + 10H2O
(NH4) 2SO4 ＋ 2NaOH = Na2SO4 + 2H2O + 2NH3 ↑
NH3 + H3BO3 = H3BO3? NH3
H3BO3? NH3 + HCl = H3BO3 + NH4Cl
Soil sample collection and processing
Determination of total nitrogen in soil (potassium dichromate-sulfuric acid digestion method)