CRF / CRH Antibody Manual

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Catalog Number: Rs-0246R

Quantity size: 0.2ml (dilute with pH 7.4 0.01 M PBS or antibody diluent)

Background: Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta . In the placenta CRH is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, CRH may act as a trigger for parturition.

Specificity: · anti- CRF is a mouse monoclonal antibody unconjugated

· Specific for CRF of human and rat

· Epitope mapping near the N-terminus of CRF of human

and rat origin

· Use for western blotting, elisa, immunoprecipitation and immunohistochemistry

· Protein G affinity chromatography purification, purity:> 95%

· Isotype: IgG

· Mol wt: 20kDa

Application: Western blotting 1: 100-500

· Immunohistochemistry 1: 100-500

· ELISA 1: 500-1000

· Optimal working dilutions must be determined by the end user.

Storage: Store at –20 ºC for one year. Avoid repeated freeze / thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01 M PBS or diluent of antibody, the antibody is stable for at least two weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.