The clinical ELISA kit measurement is usually a manual operation using a microwell strip as the solid phase measurement mode. The measurement operation is very simple, generally involving specimen collection and storage, reagent preparation, sample addition, incubation, plate washing, display In terms of color, color comparison, result judgment and result reporting and interpretation, improper any of these steps will affect the measurement results, especially the steps of sample addition, incubation and plate washing. The correct method of sample addition should be 45 degrees. The tip is added against the wall of the hole. It should be noted that the angle is too small, which will cause liquid to remain on the wall of the hole, resulting in inaccurate sample addition; the tip should be close to the wall and the Junction! At present, the clinical markers measured by serum specimens generally include antigens and antibodies of infectious pathogens, tumor markers, hormones, special proteins, cytokines and therapeutic drugs. The collection of serum samples used for the detection of antigens and antibodies, tumor markers and special proteins of infectious pathogens has no impact on time and body position. The most commonly used clinical samples for ELISA kits are serum (plasma) Sometimes, for specific testing purposes, saliva, cerebrospinal fluid, urine, feces and other specimens are also used. For the collection of serum samples used for the determination of hormones and therapeutic drugs, pay attention to the collection time or even the position may affect the measurement results. Another example is the testing of therapeutic drugs, which should be selected according to the pharmacokinetics of the most appropriate time to take blood test. As another example, when changing from a lying position to a standing position, the activity of renin in the serum will rise significantly. Such as cortisone, there will be a peak between 4 and 6 in the morning: growth hormone, luteinizing hormone (LH) and follicle stimulating hormone (FSH) are all released in an intermittent manner. Therefore, in the determination of such hormones At this time, it is necessary to take several blood samples within a closely connected time distance, and the median value is the measured value. Just consider the following aspects in handling and preservation: First of all, it is necessary to pay attention to the ELISA kit to avoid severe hemolysis. Hemoglobin contains a heme group, which has peroxide-like activity. Therefore, in the ELISA assay with HRP as the labeling enzyme, if the concentration of hemoglobin in the serum sample is high, it will be easily adsorbed during the incubation process In solid phase, it reacts with HRP substrate added later to develop color. Serum specimens can be stored at 2 ～ 8 ℃ for one week if they are separated by aseptic operation. If they are handled by bacteria, it is recommended to keep them frozen. Long-term storage of samples should be below -70 ℃. During sample collection and serum separation, care should be taken to avoid bacterial contamination as far as possible. One is the growth of bacteria, and some of the enzymes it secretes may have a decomposition effect on proteins such as antigens and antibodies; the other is some endogenous enzymes of bacteria such as E. coli β-galactosidase itself will produce non-specific interference with the measurement method labeled with the corresponding enzyme. If turbidity or flocs caused by bacterial contamination occur during specimen storage, the supernatant should be collected after centrifugal precipitation. Serum specimens stored frozen should be careful to avoid repeated freezing and thawing caused by power failure. The mechanical shear force generated by repeated freezing and thawing of the specimen will destroy the protein and other molecules in the specimen, thereby causing false negative results. In addition, the mixing of freeze-thaw specimens should also pay attention to, do not vigorously shake, just mix upside down repeatedly.
Material: High strength alloy
Color: Blue
Size: L(1.34)M*W(0.75)M*H(1.5)M
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