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Human (Col â…£) instruction manual supply, type â…£ collagen Elisa kit factory price
Human (Col Ⅳ) instruction manual, factory price experiment principle of type Ⅳ collagen Elisa kit: This kit uses the double antibody sandwich method to determine the level of human type Ⅳ collagen (Col Ⅳ) in specimens. Coat the microplate with purified human Col Ⅳ antibody to make a solid phase antibody. Add collagen Ⅳ (Col Ⅳ) to the monoclonal antibody-coated microwells in turn, and then combine with HRP-labeled Col Ⅳ antibody to form the antibody- Antigen-enzyme labeled antibody complex, after thorough washing, add substrate TMB to develop color. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the type Ⅳ collagen (Col Ⅳ) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human type IV collagen (Col IV) in the sample was calculated by a standard curve. Kit composition: kit composition 48 well configuration 96 well configuration storage instructions 1 copy 1 copy sealing film 2 pieces (48) 2 pieces (96) 1 sealed bag 1 enzyme coated plate 1 × 48 1 × 96 2 Store standard at -8 ℃: 90μg / L 0.5ml × 1 bottle 0.5ml × 1 bottle 2-8 ℃ store standard dilution 1.5ml × 1 bottle 1.5ml × 1 bottle 2-8 ℃ store enzyme label reagent 3 ml × 1 bottle of 6 ml × 1 bottle of sample diluent at 2-8 ° C 3 ml × 1 bottle of 6 ml × 1 bottle of 2-8 ° C Developer A 3 ml × 1 bottle of 6 ml × 1 bottle of 2-8 ° C Developer B solution 3 ml × 1 bottle 6 ml × 1 bottle at 2-8 ° C storage stop solution 3ml × 1 bottle 6ml × 1 bottle at 2-8 ° C Concentrated washing solution (20ml × 20 times) × 1 bottle (20ml × 30 times) × 1 bottle stored at 2-8 ℃