E. coli steps: (1) Adjust the temperature of the constant temperature water bath to 42 ° C in advance. (2) Take a tube (100μl) of competent bacteria from the -70 ℃ ultra-low temperature freezer, immediately heat it with your fingers to melt it, insert it on the ice, and place it on the ice bath for 5 ~ 10min. (3) Add 5μl of the connected plasmid mixture (DNA content does not exceed 100ng), shake gently and place on ice for 20min. (4) After gently shaking, insert into a 42 ° C water bath for 1 ~ 2min for heat shock, then quickly put it back into the ice and let stand for 3 ~ 5min. (5) Add 500μl of LB medium (without antibiotics) to the above tubes in the ultra-clean workbench and mix gently, then fix to the spring rack of the shaker and shake at 37 ℃ for 1h. (6) Take 100 ~ 300μl of the above transformation mixture in an ultra-clean workbench, and drop them into a solid LB flat Petri dish containing appropriate antibiotics, and apply evenly with a glass coating rod burned with an alcohol lamp (note: glass coating After the alcohol on the stick is extinguished, wait a while, and then apply it after it has cooled down). (7) If the carrier and host bacteria are suitable for blue and white spot screening, add 40μl 2% X-gal and 8μl 20% IPTG on the plate after the bacterial solution is dripped, and apply evenly to the glass coating rod burned with alcohol . (8) Mark the coated petri dish and place it in a 37 ° C constant temperature incubator for 30-60min until all the liquid on the surface penetrates into the medium, then put it upside down and put it in a 37 ° C constant temperature incubator overnight. (9) Spray 70% ethanol on the desktop contaminated with bacteria, dry the desktop, and write an experiment report. (10) Observe the colony colonies growing on the plate, it is better to separate the colonies from each other. Note the white plaque.
1.Material:this type of paper bag is made out of 100% natural virgin wood pulp or
recycled paper,which has no pungent odor. The raw materials which are selected
carefully and imported from USA will be dealt with special process.The imported
original wood pulp with 450 ° c high temperature sterilization is not easy to
break and tear up.All the paper we used to manufacture the paper bag is not
only super durable and reinforcement but also ECO friendly ,biodegradable,compostable
environmental-friendly,natural friendly and PLA recyclable Owing to this good
quality materials.We have very high standards on controlling raw materials.So
that the supporting weight of our paper bag holds up to higher than those at
the market. Reuse the paper bags
as many times as you like and recycle them when you are done for a greener
Earth! We offer a wide variety of material and color option to meet your
requirements.we also supply PE coated,poly or aluminum laminatived or double
glud lining inner paper,which is water proof,oil proof,grease proof,moisture
resistance,weather proof,anti-slip anti-skid,etc.
2.Size:Multi customized sizes are available,we accept personalized
sizes.Different sizes including small,medium and large measurement which are
suitable for different usages.It`s versatile.we category for kinds of
industrial usage.
3.Handles:One reasons
of this type of paper bag of excellent quality is for seeking a more luxurious
handle solution all the paper bags handles are produced by fully automatic machines.
Sturdy round twisted,flat,die cut,holes,eyelet,patch,cotton robbin,grosgrain
rope,PP rope handles of paper bags are made of 100% cellulose and fiber .They
do not come out with an ease even you pull them with hard force.These handles
are adhered with good quality super glue by fully automatic machines
instead of handmade.which looks more elegant,clear,beautiful,sanitary,flat,reinforcement
than handmade.So that it creats a smooth,elegant finish for the bags.No stray glue any where like other paper
bags do.it's more convenient and make purchase easy to carry,take
away,to go etc.
4.Bottom: Reinforcement
flat ,square rectangle,block bottom is easy to stand up ,which is neat and flat
without glue outside.We deal with the bottom carefully in order not to break
easily.You don't need to worry about stuff fall down from the paper bag and
make a trouble mess.A good quality bottom will be more useful and helpful which
is also made by fully automatic machines.It is not only solid but also
strong enough to support heavy duty weight.It can take whatever the light or
heavy stuff inside the paper bag.They are not only durable and convenient but
also environmental friendly compostable and recyclable.
5.Printing:We have the best advanced
technology fully automatic printing machines which can support up to 8 colours
printing.We accept customer designs and logos.Gravure printing,embossing
printing,debossing printing,hot stamping,screen printing,UV printing,offset printing,flexo
printing with CMYK pantone non-toxic water based ink are all available. Our
printing has a pretty good quality as the printing of the designs is not only
more durable,sun proof,weather proof but also beautiful and bright,which absorbs ink well and is
stable not easy to tide the color.We aslo supply plainpaper bag, which are perfect for craft and DIY.
Printed and embellished as you may wish. Personalize the bags to make them
uniquely yours. Print your own business logo and use them as retail carry bags.
6.Surface handling:we supply glossy and matte surface dealing.This all depends on
customer`s requirements.Whatever the glossy or matte surface makes the bag more
luxurous.
7.Appication: paper bags with kinds of
hanles are perfect for packing shoes,clothing, wine, gifts,etc.They are great suitable
for any occations and affordable for small to medium businesses, parties &
events, wedding reception and start-up business.
8.Quatity inspection systerm : One of the reasons for our good Quality is that inspection system is
best of its kind. We're proud of our quality control system. Our standards are
very high. we put great stress on quality of products.Almost every process is
computerized.. All products have to pass strict inspection before they go out.
I believe the quality is soul of an enterprise. So we won't sacrifice the
quality for quick profit.All of our products are suitable for the world market.
Our products has always come up to international standards. We have trained analysts
to do quality analysis of each process when we make the product.We also provide
quality analysis training as part of training for new workers plus a week of
futher training every year. So our products are well known for their fine
quality. All the paper bag are received a warm welcome in the world market wherever
they go.Our clients are all satisfied with them.
9.Sample:We offer free samples between
three and five days, but excluding complicate condition upon customer`s
requirnments.For instance, we charge you like 50 dollars for samples,but we
will refund you after placing an order.The expenses of samples delivery can be
nagotiated.
10.Our
factory: Jinan
Xin Shun Yuan Packing Co., Ltd is located in Ningjiabu Industrial Park,Zhangqiu
district, Jinan city, Shandong province, China.It originated in Shandong
Printing Factory (founded in 1994).It is professional manufacture of new
environmental-friendly packing bag based printing processing enterprise with
more than 12 years of experience in production and management.Recent years, our
scale is expanding. Covering an area of about 2 million square meters. And also
expand our equipment, like unit type flexo printing machines, satellite adagio
printing machines, lamination type flexo printing machines, gravureprinting ,
dry laminating machines, automatic Square Bottom Paper Bag machines, food paper
bag machines, automatic control stick bag machines, trilateral, edges the
closure, self-reliance paper bag machines, CNC cutting machines.We have 8 sets
flexo printing machines, 1 set Gravure printing machine;12 sets Paper bag
machines ; 5 sets cutting machines, tin tie machines;30 sets heat sealing
machines ; 3 sets glue coating machines , etc. We have150 staff menbers,18
management staff ,12 salesmen and 120 workers. We have ISO9001, FSC certificates.
11.Packing & Shipment:Standard export packing, 250PCS
or 500PCS each carton. We try our best to make sure the packing is
seaworth and strong enough to stand the rough handling in a long distance
shipment .In order to ensure the packing is moisture resistance,water
proof,weather proof etc.The packing corrugated cartons therefore are lined with
plastic bag from inside and strengthened double solid plastic straps.We have
rich experience on packing.We normally do not only provide air transportment
and sea transportment but also multi modal combined transportments,which is up
to customer`s requirements.
12.Payment methord: L/C,D/A,D/P,T/T,Western
Union,MoneyGram Paypal payments.
13.Our service:we are in 24/7 service,we are responsibilable for replacing the defect
products.Feel free to talk to us.
Biodegradable Paper Bags,Biodegradable Kraft Bags,Biodegradable Brown Paper Bags,Biodegradable Paper Bags Wholesale JINAN XINSHUNYUAN PACKING CO., LTD , https://www.xsy-packaging.com