Zhengzhou Weier--Comparison of several fetal calf serums commonly used in laboratories

First, special grade fetal bovine serum (the highest level, fetal bovine serum dedicated to stem cells)

1. Hyclone North American Super Fetal Bovine Serum (SH30070.03)

At present, the best performance serum, laboratory feedback is good, that is the special fetal bovine serum of Hyclone SH30070.03, Hyclone's "head card".

This serum-Hyclone special fetal bovine serum (Defined FBS), item number: SH30070, is the world's only top-grade fetal bovine serum filtered through 40 nanometers, which has excellent cell growth and product stability. The endotoxin content is less than 10 EU/ml and the hemoglobin content is less than 10 mg/dl. This serum can be widely used in various cell cultures, including the culture of any stem cell line.

2, Gibco North American premium fetal bovine serum (16000-044)

Invitrogen's original fetal calf serum produced in the United States, the number is 100 times filtered at 100nm, the endotoxin and hemoglobin content can be comparable with Hyclone's SH30070.03. It can also be used to culture most stem cell lines.

Second, excellent fetal bovine serum

1. Hyclone Canada Superior Fetal Bovine Serum (SH30396)

The most cost-effective is Hyclone Canada's excellent fetal bovine serum (Characterizend FBS), article number: SH30396, serum from Canada, collected and processed by the staff of Hyclone headquarters, collection methods, processing, calibration standards and the United States native fetal bovine serum is completely Consistently, the market price is lower than the US origin. After 3 times of 100 nm filtration, the endotoxin content is less than 25 EU/ml and the hemoglobin content is less than 25 mg/dl. This serum, before 2005, is the first choice for many laboratories to choose serum, the price is moderate, the quality is excellent, and the application cells are very extensive.

2. Hyclone Australia Superior Fetal Bovine Serum (SH30084)

Basically the same as Canada's superior serum, the level is very similar, all levels.

3. Gibco Australian fetal calf serum (10099-141) is similar to Hyclone Australian fetal bovine serum, and the market price is also about 3200 yuan / 500ml. This level of serum can also be used for stem cell culture, but it is not as good as premium fetal bovine serum.

Third, ordinary imported fetal bovine serum Hyclone South American fetal bovine serum (SV30087)

The most used Hyclone serum on the market is South American fetal bovine serum (FBS) SV30087. This serum is collected from South America, filtered and tested in China (Lanzhou). After three 100 nm filtration, the Chinese labeling method is used according to the Chinese Trademark Law. The label has an endotoxin content of 10 EU/ml or less and a hemoglobin content of 20 mg/d or less. This serum is widely used in the culture of various tumor cells. There is no data to describe it for stem cells. It is also said that a small number of people are used for stem cell culture. However, because of its high heme, it is best not to use it for stem cells. ,

Fourth, domestic series of fetal bovine serum

Domestic fetal bovine serum, there is no strict fetal bovine serum in China, fetal bovine serum itself requires puncture and blood collection, while domestic cesarean section produces fetal bovine, blood is taken for about 8 hours, and then filtered and dispensed. Therefore, domestic fetal bovine serum is not a strict fetal bovine serum. However, it is also used in many laboratories. The specific situation depends on the requirements of the experiment.

Five other serum

Newborn bovine serum, calf serum, generally domestically produced, the effect is also good, but it is best not to be used for cell culture. These serums are mostly used for blocking fluids, protective fluids, buffers, etc.

Zhengzhou Weier Biotechnology Co., Ltd. is a comprehensive enterprise integrating scientific research, production and sales. The company's business covers biological agents, chemical reagents and chemical raw materials, pre-clinical testing and consumables, elisa kits, antibodies and standards. A variety of biological products such as products and culture media.

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