Commonly known as ATP synthase, F-type ATPase, and F1F0 ATPase (F1F0 ATPase), it is the ultimate reaction of mitochondrial oxidative phosphorylation. It has a molecular weight of 500 kD and contains sixteen subunits, two of which: ATPases 6 and 8 are encoded by mitochondrial DNA. ATPase mainly has two domains: F0 is a proton channel, composed of several membrane proteins, including a, b, c, d, e, F6, A6L, OSCP (oligomycin sensitive conferring protein); and F1 catalytically active structure The domain consists of a water-soluble α3β3γδε protein. Its most characteristic enzymatic activity is oligomycin-sensitive ATP synthase. The primary function of complex V is to produce the energy ATP required for most cells. The synthesis of ATP requires a change in the proton gradient produced by the respiratory chain protein when electrons are transported to the oxygen molecules in the mitochondria. Protons are passed to the matrix via the F0 domain, activating the F1 catalytically active domain and promoting ATP synthesis. This abnormal enzyme causes myocardial and nervous system diseases. Based on ATP, under the participation of oligomycin, it is hydrolyzed by F1F0 ATPase, and then by pyruvate kinase (PK) and lactate dehydrogenase (LDH) reaction system, reduced nicotinamide adenine The converted nicotinamide adenine dinucleotide (NADH) is converted to oxidized nicotinamideadenine dinucleotide (NAD), and the change in absorbance peak (340 nm) is generated to quantitatively analyze F1F0 ATPase activity. Pu Jacket,Pu Rain Jacket,Pu Waterproof Jacket,Outdoor Disposable Rainwear Jinzhou City Hejin Clothing CO.,Ltd , https://www.hejin-cloth.com