Today is the last day of July. This month has been so fulfilled. Shanghai Hengyuan's main product, the Guinea Pig ELISA Kit, is now available. Today, Shanghai Hengyuan will look at the common problems in the experiment with you. a critical value control serum Both fixed and undetermined values. If only one quality control serum is used, it is generally set at the boundary between the normal value and the abnormal value, and is qualitatively measured at a weak positive level, called the critical value. The definition of the critical value of hepatitis B markers should be based on clinical requirements to provide a unified standard for weakly positive judgments. The critical value control serum can be used as a positive control in the kit and a third control other than the negative control. It can sensitively reflect the detection level of the kit and ensure that the specimen with weak positive reaction is not missed. Preparation of two quality control serum Each laboratory can use the quality control serum provided by the clinical center according to its own conditions, or prepare the quality control serum used in this room by the following methods. 1 Collect fresh serum that is free of hemolysis, jaundice, and no bacterial contamination. 2 56 ° C heating for 10 hours to live. 3 Remove the precipitate by centrifugation or filtration. 4 Dilute the collected serum to the desired concentration with 10% calf serum or normal human serum (PBS buffer). It is better to dilute with normal human serum because its composition is closer to the test specimen. 5 Filtering and sterilizing. Pack the small ampules in one-time use, seal them, and store them at 20 °C. Do not freeze and thaw repeatedly. The level at which the test substance is required to be detected is often considered to be the level at which the quality control serum should be selected. If there are other requirements for this test, the required concentration of the control should be added. 6 Calibration content. 20-30 test results were deleted > mean value of ± 2 SD data as a target value, and compared with known fixed serum. Use of three quality control serum The hepatitis B marker substance control serum prepared by the clinical testing center of the Ministry of Health can be kept at -20 °C for half a year. When the frozen state is melted, it should be mixed first, and the unused portion can be stored at 4 ° C for 5 days. It is not advisable to repeat ice melting or self-packing. The quality control serum required for the indoor quality control work of a certain test is generally prepared in 3-6 months. Homemade undetermined quality control serum, before a batch of quality control serum will be used up, you need to prepare the next batch of quality control serum. Quality control serum requires stable performance, the potency is unchanged in the long term, and its physical and chemical properties should be similar to the patient sample, so that it can effectively monitor. Illuminated Earth Globe,Levitating Illuminated Globe,Wood Stand Illuminated Globe,Light Up Globe With Constellations Ningbo Holly Arts & Crafts Co.,Ltd. , https://www.china-globes.com