Hefei Research Institute made new achievements in the industrialization of microbial oil

Recently, the paper "Microbial oil arachidonic acid producing bacteria ion beam mutagenesis and fermentation regulation" with Yu Zengliang and Researcher Huang Qing as the corresponding authors of the Key Laboratory of Ion Beam Bioengineering was published as a "specially invited paper" in the "Science Bulletin 》 Issue 11, 2012. The article said that Yu Zengliang's research team used ion beam mutagenesis to obtain a new strain rich in arachidonic acid oil, and achieved industrial-scale production, exploring a new way for the development of microbial oil industry.

Microbial oil is not only beneficial to health, but also a potential source of oil for biodiesel, but due to the low efficiency of the synthesis of oil by microbial cells in nature, its industrialization process is slow. Yu Zengliang's research group solved this problem well. They used ion beam biotechnology to genetically modify the lipid-containing microorganisms in the cell to obtain a new strain with fatty acid content of more than 50% of the cell biomass, of which arachidonic acid content accounted for 70% of the total lipid. Arachidonic acid is a scarce resource in foods for infants and young children, especially premature infants and non-breast-fed infants. Currently, only breast milk is abundant. At the same time, they reduced the culture medium from the original seven types to glucose and bacterial meal, and skillfully solved the safety problem caused by the fermentation substrate residue.

Yu Zengliang et al.'S research and development of microbial oils and fats is not easy. More than 20 years ago, shortly after the creation of the ion beam biotechnology platform, researcher Yu Zengliang recruited graduate students with a background in microbiology to develop and research microbial oil resources while promoting the new technology of ion beam plant breeding to the world. They took the oil-producing microorganism Mortierella alpina as a sample and genetically modified it by ion beam mutagenesis. After 5 years of efforts, the fermentation yield has been greatly improved. His student Yuan Chengling re-screened the strains for mutagenesis and fermentation conditions optimization when the original strains failed, and obtained high-yield strains with commercial value. At the end of 1999, Wuhan King Bio Engineering Co., Ltd. was established to begin industrial R & D. In the Spring Festival of 2000, Yu Zengliang led Wang Ji, Yuan Chengling and others to carry out industrial-scale synthesis research of microbial oil arachidonic acid in Wuhan's leased idle fermentation plant to produce microbial oil with the highest arachidonic acid content in the world. As the book "Single Cell Oil" by Z. Cohen and C. Ratledge says, when people are looking for alternative biosynthesis technology rich in ARA oil, Wuhan King, China Bioengineering Company uses new strains of Mortierella alpina Industrial production has been achieved.

The research by Yu Zengliang and others not only developed cheap arachidonic acid resources, but also the technologies created by them, such as ion beam cell modification technology and bacterial meal reuse, provided new ideas for solving the technical bottleneck of microbial oil industry development.

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