Difficulties in analysis of aminoglycoside antibiotics: Aminoglycoside antibiotics are a class of antibiotics containing aminoglycoside bonds. They have a broad antibacterial spectrum, have strong antibacterial activity against aerobic Gram-negative bacilli, and are widely used in clinical applications. This class of antibiotics is composed of amino sugar and basic 1,3-diaminoinositol combined with glycosidic bonds. 1,3-diaminoinositol has a basic polycyclohexanol structure, so aminoglycoside antibiotics are basic Strong and polar characteristics. At present, most of the aminoglycoside compounds are detected by liquid chromatography using a high proportion of trifluoroacetic acid as the mobile phase. When these solvents are used as mobile phases, chromatographers often find that the column efficiency of the column is greatly reduced. Poor availability, short column life and other issues. Extract from the 2010 edition of the Chinese Pharmacopoeia: Etimicin sulfate: 0.2mol / L trifluoroacetic acid-methanol 84:16; flow rate 0.5mL / min Gentamicin sulfate C component: 0.2mol / L trifluoroacetic acid-methanol 92: 8; flow rate 0.6mL / min Kanamycin sulfate: 0.2mol / L trifluoroacetic acid-methanol 92: 8; flow rate 0.6mL / min Sisomicin sulfate: 0.3mol / L trifluoroacetic acid-methanol-acetonitrile 96: 3: 1; flow rate 0.5mL / min Related substances of netilmicin sulfate: 0.2mol / L trifluoroacetic acid-methanol 84:16; flow rate 0.5mL / min Waters company solutions: Waters®'s second generation hybrid particle XBridgeTM series of chromatographic column products, by introducing an organic ethylene bridge structure during the synthesis of silica gel particles, gives it industry-leading chemical stability, pH range 1 ~ 12. At the same time improve the tolerance and mechanical strength of the chromatographic column products, the use of this series of chromatographic column products can help you solve the problem of chromatographic analysis of aminoglycoside antibiotics High-end general-purpose chromatographic column, tolerant of wide pH value, suitable for the analysis of the widest range of compounds Using Waters XBridge C18 column to analyze the chromatogram and detection results of gentamicin sulfate C component: Automated Storage And Retrieval System Automated Storage And Retrieval System,Pallet Shuttle Rack System,Mes Execution System,Mes Execution System Changchun Rochiev Intelligent Equipment Manufacturing Co., Ltd. , https://www.rochiev-en.com
